Fig. 1
From: PTHrP induces STAT5 activation, secretory differentiation and accelerates mammary tumor development
PTHrP overexpression causes alveolar hyperplasia. A Relative expression of mouse and human PTHLH mRNA in mammary gland lysates. Hprt1 was used as housekeeping gene. Bars represent mean ± SEM of fold change versus Tet-PTHrP off dox, n = 5 mice per group. B Whole-mount analysis of carmine-stained number 4 inguinal mammary glands from mice at 5 and 13 weeks. Scale bar 5 mm. C H&E stained cross-sections from 13-week-old mice. Scale bar 100 µm. D Representative images and quantification of EdU incorporation in sections of mammary glands from mice at 5 weeks of age. Bar graphs represent the percentage of Edu-positive cells over a minimum of 1000 total nuclei (DAPI). N = 3 mice per group, ****p < 0.0001 **p < 0.01