Skip to main content
Fig. 2 | Breast Cancer Research

Fig. 2

From: Selective pressure of endocrine therapy activates the integrated stress response through NFκB signaling in a subpopulation of ER positive breast cancer cells

Fig. 2

Single-cell RNA sequencing identifies an NFκB + cell population. a scRNA sequencing was conducted on MCF-7 cells treated with 4OHT for 2 weeks in clonogenic conditions. Bi-dimensional representation of 648 single-cell transcriptomes is shown (UMAP). b Box plots represent the Hallmark NFκB signature pathway score (median, interquartile values, range, and outliers) for each cell cluster. FEA for each cluster was performed but threshold for enrichment was not reached for either (Cluster 0: AUC 0.56, P = 0.02; Cluster 1: AUC 0.44, P = 0.02). c Bar plot showing the distribution of z-scores for the Hallmark NFκB signature on a per cell basis. d NFκB + cells (green) and NFκB- cells (gray), based on individual cell z-scores, are distributed across cell clusters defined in a. e FEA was performed for additional known NFκB genes signatures from MsigDB. AUC values are shown in a heatmap, and P-values are presented in Additional file 2: Table S1

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us