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Fig. 7 | Breast Cancer Research

Fig. 7

From: Quantitative glycoproteomics analysis identifies novel FUT8 targets and signaling networks critical for breast cancer cell invasiveness

Fig. 7

Core fucosylation sites of IL6ST and its biological function. a Schematic diagram of human IL6ST wild type (WT) and its core fucosylation site mutant IL6ST-7NQ. TM, transmembrane domain. b Western blot and LCA blot analysis of immunoprecipitated FLAG.IL6ST-WT and FLAG.IL6ST-7NQ. cf Effect of IL6ST core fucosylation site mutant on the migratory and invasive ability of breast cancer cells. Cell migration (c, d) and invasiveness (e, f) of IL6ST-KO MDA-MB-231 (c, e) and Hs578T cells (d, f) re-expressing FLAG.IL6ST-WT or FLAG.IL6ST-7NQ were measured by Transwell assay with (invasion assay) and without Matrigel coating (migration assay). Data are mean ± SD. **p < 0.01. OD optical density

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