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Fig. 6 | Breast Cancer Research

Fig. 6

From: Quantitative glycoproteomics analysis identifies novel FUT8 targets and signaling networks critical for breast cancer cell invasiveness

Fig. 6

Establishment of IL6ST-knockout cells by CRISPR-Cas9-mediated genome editing. Two independent CRISPR-Cas9 clones targeting exon 3 and 8 of IL6ST were established (KO#1 or #2) in two invasive breast cancer cells, MDA-MB-231 (a) or Hs578T (b), respectively. Depletion of IL6ST gene were validated by western blot. kDa, kiloDalton. Cell migration (c, d) and invasiveness (e, f) of control and IL6ST-KO MDA-MB-231 (c, e) and Hs578T cells (d, f) by Transwell assay with (invasion assay) and without Matrigel coating (migration assay). Data are mean ± SD. **p < 0.01, IL6ST-KO versus parental cells. OD optical density

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