Fig. 4From: Quantitative glycoproteomics analysis identifies novel FUT8 targets and signaling networks critical for breast cancer cell invasivenessValidation of the SILAC results of identified glycoproteins. Core fucosylation of the selected proteins was eliminated by FUT8 knockout (KO). Recombinant proteins in the control or two FUT8-KO MDA-MB-231 cell lines were probed with biotinylated LCA, then detected with streptavidin-conjugated horseradish peroxidase. Protein domain organization of these selected proteins was depicted according to the Uniprot database (https://www.uniprot.org); potential N-linked glycosylation sites are marked. TM transmembrane domainBack to article page