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Fig. 4 | Breast Cancer Research

Fig. 4

From: Quantitative glycoproteomics analysis identifies novel FUT8 targets and signaling networks critical for breast cancer cell invasiveness

Fig. 4

Validation of the SILAC results of identified glycoproteins. Core fucosylation of the selected proteins was eliminated by FUT8 knockout (KO). Recombinant proteins in the control or two FUT8-KO MDA-MB-231 cell lines were probed with biotinylated LCA, then detected with streptavidin-conjugated horseradish peroxidase. Protein domain organization of these selected proteins was depicted according to the Uniprot database (https://www.uniprot.org); potential N-linked glycosylation sites are marked. TM transmembrane domain

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