Table 1 Sequences for primers used in the real-time PCR assays and guide RNA sequences and screening primer sequences for CRISPR experiments
From: INHBA is a mediator of aggressive tumor behavior in HER2+ basal breast cancer
Primers | Sequence (5′ to 3′) | Purpose |
|---|---|---|
AAGTCGGGGAGAACGGGTATG | RT-qPCR | |
INHBA-R | TCTTCCTGGCTGTTCCTGAC | RT-qPCR |
B2M-F INHBA-F | TGCTGTCTCCATGTTTGATGT | RT-qPCR |
B2M-R | TCTCTGCTCCCCACCTCTAAGT | RT-qPCR |
sgRNA275 FOR | CACCGCGCGATCAGAAAGCTTCATG | Guide RNA for CRISPR |
sgRNA275 REV | AAACCATGAAGCTTTCTGATCGCGC | Guide RNA for CRISPR |
sgRNA95 FOR | CACCGCGCACAGGACGGACAGTCGG | Guide RNA for CRISPR |
sgRNA95 REV | AAACCCGACTGTCCGTCCTGTGCGC | Guide RNA for CRISPR |
g95-surveyor-F1 | ACAGCCACAAACCTACAGCAC | Competition-based PCR |
g95-surveyor-R1 | TCCACATACCCGTTCTCCCC | Competition-based PCR |
g95-surveyor-F2 | CCCTTGCTTTGGCTGAGAGG | Competition-based PCR |
g95-surveyor-R2 | CAATGCCAGCACCAACCTGA | Competition-based PCR |
g275-surveyor-F1 | TCAGCCAGAGATGGTGGAGG | Competition-based PCR |
g275-surveyor-R1 | GTGTGACCCGCTGGGTTTAG | Competition-based PCR |
g275-surveyor-F2 | GATGCCCTTGCTTTGGCTGA | Competition-based PCR |
g275-surveyor-R2 | CAATGCCAGCACCAACCTGA | Competition-based PCR |
g95-F-in | AGCGCGGCCCCCGACT | Competition-based PCR |
g95-R-in | GCACAGGACGGACAGTCGG | Competition-based PCR |
g275-F-in | GAACGCGATCAGAAAGCTTCATG | Competition-based PCR |
g275-R-in | CCCGACTTTGCCCACATGAA | Competition-based PCR |