Fig. 8
From: INHBA is a mediator of aggressive tumor behavior in HER2+ basal breast cancer
A Growth curves for 21-MT1 cells with INHBA deleted by CRISPR targeting. Px459 cells went through the selection process but no guide RNA was added and serves as a control. The sg275(2B-12) and sg95(2A-11) CRISPR-treated clones both showed frameshift mutations that altered the coding sequence of the gene. The sg275(2B-3) and sg275(2C-1) CRISPR-treated clones showed in-frame deletions of 2 amino acids, resulting in maintenance of one copy of the protein sequence. Examination of growth rates showed that the clones with effective CRISPR knockout of INHBA grew significantly slower than the controls, as did in-frame CRISPR mutants. Error bars are ± S.D. (* indicates p < 0.05 compared to px459(A5) controls; ** indicates p < 0.001 compared to px459(A5) controls). B Treatment of 21-MT1 2A11 clone with 100 ng of an INHBA cDNA increases growth rescue compared to GFP control plasmid-treated cells (p < 0.001)