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Fig. 4 | Breast Cancer Research

Fig. 4

From: INHBA is a mediator of aggressive tumor behavior in HER2+ basal breast cancer

Fig. 4

A Knockdown of INHBA sensitizes 21-MT1 cells to lapatinib as shown by % growth inhibition. Treatment with Q4 (green line) or Q6 (red line) siRNA resulted in both lower growth (not shown), as expected. Q4 siRNA increased sensitivity to lapatinib resulting in a left-shift of the curves compared to scramble-treated control (blue line; p < 0.001), while Q6 showed a trend but failed to reach significance (p = 0.16). In particular, note the differential sensitivity of the cells to 5 µM lapatinib (arrow). B Growth of 21-MT1 cells in 3-d Matrigel is inhibited by knockdown of INHBA, but has no effect on the growth of SKBR3 cells. C Quantification of growth and response of SKBR3 and 21-MT1 cells in 3-d Matrigel. INHBA knockdown significantly inhibits growth of 21-MT1 cells but lapatinib has minimal effect. However, 21-MT1 cells are more sensitive to lapatinib when INHBA is knocked down. In contrast, INHBA knockdown has no effect on the growth of SKBR3 cells, but lapatinib significantly impairs the growth of these cells. Error bars are ± Standard Error of the Mean (SEM)

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