Fig. 7
From: Microtubule disruption reduces metastasis more effectively than primary tumor growth
Vinorelbine drug response in tumor cells recovered from blood samples. MDA-MB-231 TD cells captured using ANGLE Parsortix and VTX-1 systems. a Parsortix schematic of CTC staircase capture method from whole blood. Live DIC images to visualize cell capture and CellMask orange stained cells to visualize McTNs (arrows). b MDA-MB-231 TD cells (1 × 103) spiked into 7.5 ml whole blood were recovered in Parsortix cassette, eluted onto microfluidic cell tethering slides and imaged live after DNA staining with Hoechst and CellMask Orange cell membrane dye. c Confocal microscopy of MDA-MB-231 tumor cells (5 × 103) isolated from 7.5 ml whole blood using the VTX-1 system and then treated with Vinorelbine (10 μM, 1 h) or drug vehicle (0.1% DMSO, 1 h) before elution onto microfluidic cell tethering slides, chemical fixation and Hoescht (blue)/WGA (red) staining. Images show an overlay of each color. Arrows show McTN protrusions on isolated tumor cells. d McTN analysis of the cell body outline (blue), cell perimeter (red), and McTNs tips (yellow) of fixed and tethered cells. e McTN analysis measuring average number McTNs/cell after treatment with vehicle or Vinorelbine. f Analysis of average distance of McTN tips from cell body boundary for cells treated with vehicle or Vinorelbine. For cell isolation and fixation analysis a total of 78 cells in the vehicle control population and 80 cells for the Vinorelbine treated population was analyzed from three independent experimental replicates