Fig. 6

Lung retention and metastatic development after Vinorelbine treatment. a Representative bioluminescence images of mice treated with vehicle control (0.1% DMSO) or 5 mg/kg Vinorelbine at 24 h and 2 h prior to injection of MDA-MB-231 TD cells. The vehicle control population resulted in 15 out of 17 animals (88%) exhibiting tumor formation in lung tissue between 3–12 weeks post inoculation with 2/17 (12%) surviving. The focused Vinorelbine treatment resulted in 10 out of 19 animals (53%) with disease-free survival at 30 weeks and 9 out of 19 animals (47%) with delayed tumor formation. Photon flux color scale is shown to the right. b Fold differences of retained bioluminescence in the lung of each mouse inoculated with MDA-MB-231 TD cells via tail vein. Data represent individual animal examined and measured as a fold change of the initial value of each independent animal. c Representative images of immunohistochemistry for human mitochondria and hematoxylin and eosin (H&E) staining performed at ethical end-points. Images captured at a magnification of 20 × indicate metastatic burden, scale bar, 200 μm. d Overall survival was assessed by Kaplan–Meier analysis (Wilcoxon test P < 0.001 and Log-rank test P < 0.001) for mice treated with Vinorelbine