Fig. 4

Vinorelbine treatment decreases homotypic cluster aggregation in vitro. MDA-MB-231 TD, MDA-MB-436 TD and BT-549 TD cells were treated in attached conditions for 1 h in Vinorelbine (10 μM). Cells were trypsinized, counted, and plated in a low attach plates for 4 h to test aggregation. At given timepoints, cells were tethered onto a TetherChip surface and stained with the nuclear dye Hoechst 33258 (1:5000). a Representative images of control and Vinorelbine-treated MDA-MB-231 TD, MDA-MB-436 TD and BT-549 TD cells over time to visualize cluster formation efficiency. Images taken of tethered and stained cells at t = 0 and 4 h. at a 4 × magnification. Scale bar = 100 µm. b Analysis measuring the efficiency of clustering by comparing the number of individual clusters over time. Individual values at t = 0 h were divided by respective experimental final cluster numbers (t = 4 h) for each condition