Fig. 2

Vinorelbine increases acetylated α-tubulin while decreasing filamentous microtubule network. a Immunoblot analysis of Vinorelbine (10 μM) treated MDA-MB-231 TD cells results in an increase in acetylated α-tubulin (acetyl-tubulin) whereas detyrosinated tubulin (Glu-tubulin) and total α-tubulin remain unchanged at 24 h and 48 h, compared with vehicle control (0.1% DMSO). Vinorelbine (10 μM) yielded minimal PARP cleavage at 24 h. However, by 48 h., approximately 50% PARP cleavage is observed. b Immunofluorescence shows filamentous tubulin (green) is destroyed within 15 min of 10 μM Vinorelbine treatment, as well as the organization of acetylated (Ac) and detyrosinated (Glu) microtubules. Analysis at additional times (data not shown) reveals the continued absence of filamentous tubulin. Hoechst was used to visualize the nuclei (blue). Images taken at 60 × magnification. Scale bar = 20 µm