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Fig. 1 | Breast Cancer Research

Fig. 1

From: Microtubule disruption reduces metastasis more effectively than primary tumor growth

Fig. 1

Vinorelbine decreases McTNs on breast tumor cells. MDA-MB-231 TD cells stained with CellMask Orange cell membrane dye, detached and suspended in media containing vehicle (0.1% DMSO) or Vinorelbine (10 μM) for 1 h. a Representative confocal images (top) and inverted epifluorescence images (bottom) of free-floating cells on a low attach plate. Scale bars correspond to 10 μm. b Vinorelbine caused a significant decrease in McTN frequency (%) compared to vehicle control. McTN scoring consists of mean values from four independent experiments where 100 cells were blindly counted and averaged. c Representative raw images of tethered cells and computer determined cell body outline (blue), cell perimeter (red), and McTNs tips (yellow). d Live cell analysis measuring an average number of McTN tips for cells treated with vehicle (0.1% DMSO) or Vinorelbine (10 μM) for 1 h. McTN number is the number of McTNs per cell. McTN distance is average distance of McTN tips from cell body boundary. For live cell image analysis, a population of 25 cells per condition was analyzed from 3 independent experimental replicate

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