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Fig. 3 | Breast Cancer Research

Fig. 3

From: LncRNA IPW inhibits growth of ductal carcinoma in situ by downregulating ID2 through miR-29c

Fig. 3

miR-29c is downregulated in DCIS and is controlled by IPW. A RNAs were isolated from MCF10A and DCIS.com cells and subjected to Affymetrix microRNA array. Differentially expressed miRNAs based on p-value (< 0.05) were plotted as a heat map. B miR-29c expression in MCF10A and DCIS.com was quantified by qRT-PCR (TaqMan probes, n = 5/group). C miR-29c expression was examined in HMEC and SUM225 cells by TaqMan qRTPCR (n = 5/group). D miR-29c expression was examined in DCIS and matching normal tissues used for Fig. 1A by TaqMan qPCR (n = 8/group). E DCIS.com cells were transduced with either empty vector or miR-29c-expressing vector and miR-29c expression was examined by TaqMan qRT-PCR (n = 5/group). F DCIS.com cells were transduced with either empty vector or miR-29c-expressing vector. Tumor initiating stem cell (CD24low44highESAhigh) population in was quantified by flow cytometry (n = 4/group). G DCIS.com (control) and DCIS.com-miR-29c cells were seeded in ultra-low attachment plate supplemented with mammosphere media (1000 cells/well, n = 5/group). Number of spheres were calculated at day 5 and visualized under the microscope. H IPW and miR-29c expression were examined in RNA samples isolated from DCIS tumor sections by qRT-PCR (n = 10/group). Correlation between IPW and miR-29c is shown. The degree of correlation was inferred from Pearson r and p value. I, J miR-29c expression was examined by TaqMan-based qPCR in empty vector or IPW expressed DCIS.com (I) and SUM225 (J) cells (n = 5/group). miR-361-5p was used as internal control. Statistical inference unless otherwise specified was determined by unpaired two tailed Students t test. Data is represented as mean + S.E.M. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)

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