Fig. 2
From: Gene expression predicts dormant metastatic breast cancer cell phenotype
Isolation of dormant PyMT-Bo1 cells from bone for scRNA-seq. a Schematic of the experimental timeline for dormant PyMT-Bo1 isolation. b Dilution of DiD membrane dye fluorescence following PyMT-Bo1 cell division in vitro. c Gating strategy for FACS sorting of DiD + and DiD- PyMT-Bo1 cells for scRNA-seq. d Fluorescence image of sorted DiD + PyMT-Bo1 cells. (Red: mApple + tumor nucleus; Cyan: DiD fluorescence; Scale bar = 10 µm) e Fluorescence image of dormant DiD + PyMT-Bo1 cells in situ in the bone. (Red: mApple + tumor nucleus; Cyan: DiD fluorescence; Blue: DAPI; White arrow points to a DiD + tumor cell, Scale bar = 80 µm), f Tracking sorted DiD + PyMT-Bo1 cell proliferation in vitro over time as indicated on the timeline. (Scale bar = 100 µm)