Fig. 1
From: Gene expression predicts dormant metastatic breast cancer cell phenotype
Development of an efficient tumor cell isolation system for DTCs in the bone. a PyMT-Bo1 labeling strategy using Thy1.1, H2B-mApple and Luc2. b Flow cytometry analysis comparing tri-labeled PyMT-Bo1 tumor cells admixed with murine wildtype bone marrow cells. c BLI image of WT C57BL6/J mice 9 days after IC injection of 500,000 PyMT-Bo1 cells. Scale represents photon flux (photons/sec/cm2/sr). d Representative ex vivo BLI image comparing PyMT-Bo1 cells spun from the bone marrow versus those that remain attached to the bone. Scale represents photon flux (photons/sec/cm2/sr). e Two-photon microscopy image of tumor-bearing femur. 3D reconstruction size: 425 × 425 × 168 µm3 (Red: mApple + tumor cell nucleus; Gray: second hormonic generation of collagen fibers) f Summary of PyMT-Bo1 signal in different fractions of the bone after 3 rounds of collagenase digestion. g Flow cytometry analysis of PyMT-Bo1 enrichment before and after Thy1.1 MACS. h Schematic of final tumor cell isolation strategy from bone