Fig. 3

Bovine pituitary extract is the active component repressing Postn expression. A NeuNDL cells were seeded and grown in MEGS-depleted medium for up to 48 h, and POSTN expression was assessed at multiple time points. β-Actin was used as a loading control. B NeuNDL cells were seeded and then grown in either MEGS, or individual components of MEGS (EGF 3 ng/ml, IFG1 0.01 µg/ml, hydrocortisone 0.5 µg/ml, BPE 0.4% v/v), and Postn protein levels and mRNA expression C were assessed after 24 h. mRNA expression data are represented as mean ± SEM. N = 3, **P ≤ 0.01