Fig. 3

Deletion of STAT5 in myeloid cells enhances lung metastasis. A Immunoblot for total STAT5 in macrophages isolated from 4T1 tumors in fl/fl or cKO mice. Densitometry analysis relative to loading control. B Flow cytometric analysis of the relative frequency (among CD45 + cells) and total number of macrophages isolated from 4T1 tumors at endpoint. C Kaplan–Meier curves of 4T1 cells or E HC11/R1-LM cells transplanted into STAT5^fl/fl (n = 5–13) or STAT5^cKO (n = 8–11) mice. % Survival on Y-axes indicates proportion of mice reaching tumor size endpoint of 1cm³. D Lung sections from 4T1 and F HC11/R1-LM tumor-bearing mice stained for H&E and quantified percent metastatic area per tissue section. Lungs quantified from at least 4 mice per group. Unpaired t-test was used for statistical analysis. *P < 0.05, **P < 0.01. Scale bar: 50 μm