Fig. 3From: Secreted indicators of androgen receptor activity in breast cancer pre-clinical modelsExpression of PSA, ZAG and PIP is regulated by AR in breast cancer cell lines. Protein extracts of whole cell lysates were examined by western blot after 4-days cultured with vehicle control, DHT (10 nM), DHT (10 nM) plus Enza (20 μM) or Enza (20 μM). Each band was quantified by densitometry and the expression levels of PSA (a), ZAG (b) and PIP (c) relative to GAPDH normalized to vehicle control are shown for representative cell lines. Data for the remaining cell lines are shown in Additional file 3: Fig. S3a–c. d AR-dependent regulation of candidate protein expression in all tested cell lines are summarizedBack to article page