Fig. 2

Gene expression of KLK3, AZGP1 and PIP is regulated by AR in MDA-MB-453 cells. a AR binding on gene loci was assessed by AR-ChIP-seq in MDA-MB-453 breast cancer cells. Gene locus for each candidate gene and read depth for each binding are shown. Red arrows indicate significant AR-binding peaks detected, and black arrows indicate known enhancer regions containing the AR binding motif. b Expression of each candidate gene in MDA-MB-453 cells was examined by qRT-PCR after 4-days cultured in indicated conditions. Expression levels were normalized to 18S rRNA; mean ± standard deviation, ***p < 0.001