Fig. 2

Effect of E2 supplementation on immune humanization in mice. a Experimental timeline. 6–7 weeks old NSG-SGM3 female mice were injected with 20 mg/kg/day busulfan on two consecutive days. Five days later, mice were IV injected with 85,000 hCD34⁺ HSCs and 24 h later received E2 supplemented acidified drinking water for 3.5 weeks (n = 5, HSC donor ID 7734, KIR-mismatched to HCI-013). Control mice were kept on unsupplemented acidified drinking water. Mice were harvested 13.5 weeks post-humanization, at which point BM and blood were freshly isolated, and flow staining was performed. Flow cytometry was performed on the day of tissue harvest without fixation. b Flow cytometry analysis of hCD45⁺ cells in BM, and blood of humanized mice receiving E2 or unsupplemented acidified drinking water are shown as percent of viable cells (n = 2–4). c Flow cytometry analysis of hCD11b⁺, hCD3⁺, hCD56⁺ and hCD19⁺ cells in BM and blood of humanized mice receiving E2 or unsupplemented acidified drinking water are shown as percent of hCD45⁺ cells (n = 2–4)