Fig. 3

Abundance and distribution of EPH groups across IHC-surrogate subtypes of breast cancer. A Table showing the eight EPH groups of single cell classification according to thresholding of MxIF signal intensities of ER, PR and HER2. Ki67 positivity was also determined in each cell and denoted by shaded color bars. B Proportion of EPH groups (EPH1-8) in tumor cores that were classified as Luminal A-like (Number of cores, N = 24), Luminal B-like (HER2-) (N = 36), Luminal B-like (HER2+) (N = 6), HER2+ (non-luminal) (N = 13) and triple-negative (TNBC) (N = 22). Cores taken from the same cancer are aligned in pairs for comparison. Spot_032 and spot_059 are paired cores but assigned to different IHC-surrogate subtype and marked with asterisks (**). C Boxplot presentations of the percentages of cells of each EPH group that are either Ki67-negative (top panels) or Ki67-positive (bottom panels) across IHC subtypes. LumBH2- LumB, HER2+. To indicate subtypes that showed significant difference (p = 0.01) for a EPH group, the subtypes are marked with dashed lines with the major subtype denoted with an asterisk (*)—for EPH Group 1 (Ki67-), significant difference was found between TNBC and LumA, TNBC and LumB, and TNBC and LumBH2, respectively. Double asterisk (**) denotes the subtype that showed significant difference with all other subtypes—for EPH Group 2 (Ki67 +), significant difference were found between HER2 and LumA, HER2 and LumB, HER2 and LumBH2, and HER2 and TNBC