Fig. 2

Distribution of MxIF signal intensities of P53, P16 and P21 from cores with various IHC patterns. A Comparison of P53 staining pattern from MxIF and IHC of representative cores which were classified as normal, null or overexpressed (OE) with IHC scoring. Virtual H&E image of the corresponding core in MxIF is shown in inset. A small region of each image was zoomed in 40 × magnification (dotted circle). B Boxplot graphs showing MxIF P53 normalized signals from single cells in cores classified as P53 normal, null or overexpressed (OE) with IHC evaluation (top). The plot is further magnified (bottom). The number of cores (N) included in each group is as indicated. C P16 evaluation of MxIF and IHC staining of cores that were classified as normal or overexpressed (OE) in IHC. D Boxplot graphs showing MxIF P16 normalized signals from single cells in cores that were classified as normal or overexpressing (OE) P16. E P21 IHC were scored with the percentage of positive cells and average intensity. Representative cores with negative staining (neg), 40% weak (40-w), 80% moderate (80-m) and 90% strong (90-s) are shown, together with MxIF staining of the same core. F Boxplot graphs showing P21 MxIF normalized signals from single cells in cores with the indicated IHC scores