Fig. 1

Comparison of MxIF biomarker signal intensities to IHC scoring on serial sections. A, C, E Paired MxIF with vH&E in inset (top) and IHC (bottom) images of ER (A), PR (C) and HER2 (E) of representative cores are shown. A small region of each image is zoomed in 40 × magnification (dotted circle). The corresponding IHC score for each core is as indicated. For ER and PR, IHC were scored with the percentage of positive cells and average marker intensity. For example, 90-w indicates 90% positive cells with weak staining. m-Moderate staining, s- Strong staining. B, D, F The distribution of normalized MxIF signal intensities from single cells in cores that were classified based on their corresponding IHC staining on serial sections for ER (B), PR (D) and HER2 (F). Normalized MxIF values (ERnorm, PRnorm or HER2norm) from single cells were compared between cores where IHC scoring indicated that they are either negative (Neg), or a majority (60% +) of cells were scored as weak (Low), moderate (Med), or strong (High) for ER and PR, and negative, 1+, 3+ for HER2. The number of cores (N) included in each group is as shown. G Ki67 comparison. Paired MxIF and IHC of representative cores with the indicated IHC scores of positive percentages are shown. H Distribution of normalized Ki67 MxIF signal intensities from the same cores