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Fig. 4 | Breast Cancer Research

Fig. 4

From: Fluid flow exposure promotes epithelial-to-mesenchymal transition and adhesion of breast cancer cells to endothelial cells

Fig. 4

a Smad2 protein expression (total SMAD2 and pSmad2-linker) as determined by western blotting in MDA-MB-231 cells. b Quantitative RT-PCR analysis of SNAI2 expression in MDA-MB-231 cells transfected with control (–), Smad2 or Smad3 siRNA and stimulated with fluid flow for 20 h. Control siRNA (negative control) consisted of a scrambled sequence. Fold changes were calculated using the comparative cycle threshold method and normalized to expression in unstimulated controls. Statistical significance was determined using the Student’s t-test and the threshold was set at p < 0.05. Duplicate wells were run for every sample and beta-2-microglobulin was used as the reference gene. Results in the graphs are expressed as mean ± standard error of the means (*, p < 0.05) from at least three samples for each condition

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