Fig. 2
Oestrogen deprivation upregulates cytokine secretion in MCF-7 human breast cancer cells. a CCL5, b TNF, c IL-18, d CCL22 and e IL-6. MCF-7 cells were cultured with (+E2) or without (− E2) 1 × 10−9 M oestradiol, and cell supernatant was collected at 0, 24, 72 and 120 h and analysed with a multiplex assay. Data are normalised to the number of cells in each well. Error bars are SEM based on nine biological replicates performed across three independent experiments. P-values were calculated using unpaired t-tests