Fig. 2

Percentage of specified epithelial 3D-growth patterns of EpH4 cells cultured within Matrigel (Ctrl) or co-cultured with mammary fibroblasts from 3-days pregnant (P3) or age-matched adult virgin mice (V12). Bars represent standard errors (n = 3 replicate experiments). Images below show representative images for each 3D structure classification of small spheroids (left), small branched (more elongated; 2^nd from left), large spheroids (at least 5-times larger than small spheroids; middle), large branched (branched but compact; 2^nd from right) to large highly branched structures (branched and extended; right). Scale bars are 50 µm. The p-value (0.001) shows the statistically significant difference in the number of large, highly branched structures between P3 and virgin control (Student T-test)