Fig. 2

TAZ is essential for telomere maintenance in TNBC cells. A Western blot analysis in the indicated MDA-MB-231 cells to indicate the depletion efficiency of TAZ expression. Quantification of Western blots is shown in Additional file 1: Fig. S5C. B Telomere-specific qPCR analysis in the indicated MDA-MB-231 cells to determine the relative telomere length. C Southern analysis of TRFs in the indicated MDA-MB-231 cells to determine the relative telomere length. D Western blot analysis in the indicated BT549 cells to indicate the protein levels of TAZ. Quantification of Western blots is shown in Additional file 1: Fig. S5D. E Telomere-specific qPCR analysis in the indicated BT-549 cells to analyze the relative telomere length. F Southern analysis of TRFs in the indicated BT549 cells to determine the relative telomere length. G Western blot analysis in BT549 cells overexpressing wild type TAZ and its activated mutant (TAZ-4SA) with antibodies as indicated. Quantification of Western blots is shown in Additional file 1: Fig. S5E. H Telomere-specific qPCR analysis to determine the relative telomere length in the indicated BT549 cells. I Western blot analysis in T47D cells overexpressing wild type TAZ and its activated mutant (TAZ-4SA) with antibodies as indicated. J Telomere-specific qPCR analysis to determine the relative telomere length in the indicated T47D cells. K Western blot analysis in MCF10A cells overexpressing wild type TAZ and its activated mutant (TAZ-4SA) with antibodies as indicated. L Telomere-specific qPCR analysis to determine the relative telomere length in the indicated MCF10A cells. Data are presented as mean ± SEM. At least three repeats were carried out for each test. The p values were determined by one-way ANOVA followed by Tukey’s multiple-comparisons. NS, not significant, *p < 0.05, **p < 0.01, ***p < 0.001