Fig. 1
From: Evaluation of FGFR targeting in breast cancer through interrogation of patient-derived models
FGFR expression and phosphorylation signatures in human breast cancer PDX as determined by MS-based tyrosine phosphorylation profiling and immunoblotting. a Unsupervised hierarchical clustering of PDX based on 115 identified tyrosine-phosphorylated kinase peptides. Relative abundance is based on z-score across the 19 PDX samples. The luminal-B PDX HCI-009 is indicated by an asterisk. b Site-selective phosphorylation of specific FGFRs based on z-score across the 19 PDX samples. Gray shading indicates that the FGFR phosphorylation site was undetectable by MS. c Expression of specific FGFRs across the panel. Protein lysates from 17 PDX samples were immunoblotted with the indicated antibodies. Total cell lysate indicates lysates from specific TNBC cell lines used as positive controls for the respective antibodies (CAL120 for FGFR1, MFM-223 for FGFR2, SUM185PE for FGFR3 and MDA-MB-453 for FGFR4)