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Fig. 5 | Breast Cancer Research

Fig. 5

From: Sensitivity to targeted therapy differs between HER2-amplified breast cancer cells harboring kinase and helical domain mutations in PIK3CA

Fig. 5

PIP3 signaling remains high in H1047R knockin but not E545K knockin cells after lapatinib treatment. A Confocal imaging of cells after 36 h of treatment with DMSO or lapatinib (500 nM). PIP3 staining can appear in clusters (yellow arrows, top left image). B Quantification of PIP3 staining in cells using CellProfiler. Cell boundary was determined by phalloidin stain (not shown) and PIP3 stain overlapping with phalloidin was calculated. Sum of pixel intensity of PIP3 divided by the area overlapping with phalloidin was calculated for every cell per image. Average values across 15 images are shown in the graph. Error bars represent standard deviation. * and ** indicates PIP3 intensity values for lapatinib-treated cells are significantly higher than untreated matched samples (* indicates p < 0.005 and ** p < 0.001)

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