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Fig. 2 | Breast Cancer Research

Fig. 2

From: Sensitivity to targeted therapy differs between HER2-amplified breast cancer cells harboring kinase and helical domain mutations in PIK3CA

Fig. 2

Analysis of signaling pathways showing H1047R knockin cells maintain intracellular signaling in presence of lapatinib. A Whole cell lysates collected after 48 h of exposure to 500 nM lapatinib or vehicle were separated by gel electrophoresis and analyzed for the indicated proteins. H1047R knockin cells retain high levels of phospho-AKT and downstream substrates phospho-PRAS40 and phospho-S6 (red boxes) whereas E545K knockin cells do not. B Cells were treated with 500 nM lapatinib for a timecourse of 72 h. Whole cell lysates were collected at the indicated times and separated by gel electrophoresis and analyzed for the indicated proteins. H1047R knockin cells show recovery of phospho-AKT levels and persistent phosphorylation of S6 which is not seen in E545K knockin cells

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