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Fig. 3 | Breast Cancer Research

Fig. 3

From: Progesterone receptor antagonists reverse stem cell expansion and the paracrine effectors of progesterone action in the mouse mammary gland

Fig. 3

PR regulated induction of EMT-associated genes in the mouse mammary gland. a MaSc, LP, and LM cells were examined for the induction of EMT-associated genes in RNA seq data. b–g qRTPCR was performed to confirm the mRNA expression and Livak’s fold change was calculated relative to MaSC (Sham). b Cdh1 expression was significantly repressed in EP-treated MaSC (p < 0.01), LP (p < 0.01), and LM (p < 0.001) subpopulations, which was reversed by treatment with EP + PR inhibitors (p < 0.0001). c Snai1 showed significantly increased expression in all three subpopulations (p < 0.01) in EP-treated group relative to sham and significantly decreased expression in mice treated with EP+ PR inhibitors (p < 0.001). d Zeb1 was induced in the EP-treated mice relative to sham-treated mice in the MaSC (p < 0.01) and LP and LM compartments (p < 0.0001). EP+ PR inhibitor-treated mice show a repression of this induction in both luminal (LP, p < 0.0001; LM, p < 0.01) and basal compartments (p < 0.0001). e Zeb2 was also significantly induced upon exposure to EP in MSC (p < 0.001), LP (p < 0.0001), and LM (p < 0.0001) relative to sham. Mice treated with EP + PR inhibitors showed a significantly lower expression of Zeb2 relative to EP in MSC and LP cells. LM cells showed an induction of Zeb2 expression in EP + TPA (p < 0.001) and EP + MFP mice (p < 0.0001). f Ptch2 shows a significant induction in the EP-treated mice compared to the sham group in all the three subpopulations LP (p < 0.05), LM (p < 0.0001), and MaSC (p < 0.0001). Treatment with EP + PR inhibitors shows a reversal of this effect with significant downregulation observed in all three lineages (p < 0.0001). g Gli3 expression in the EP group relative to sham also show an upregulation in MaSc (p < 0.0001) and LP (p < 0.0001) cells but not in LM cells. The induction of this effect was reversed after treatment with EP + MFP (p < 0.0001) and EP + TPA (p < 0.0001)

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