Fig. 7

Dox induction of Fyn is dependent on ATR but not Topoisomerase II. MCF7 cells were treated with siRNA, vehicle (DMSO), and 0.4 μM Dox as shown. A–C Cells treated with AS negative control, ATM, or ATR siRNA were analyzed for A protein by immunoblot for ATM, ATR, and Actin. B Fyn and C Yes expression by qRT-PCR analysis (Mean ± SEM, *p < 0.05, n = 5). D–F Cells treated with AS negative control, Top2A-siRNA, or Top2B-siRNA were analyzed for D Top2A knockdown by immunoblot and E Top2A and Top2B; and F Fyn by qRT-PCR. For all analyses, actin was used as a reference gene. Data is presented as mean normalized expression (Mean ± SEM, n = 3)