Fig. 3

Sublethal Dox treatment induces migration without inducing an EMT or increasing the CSC population. MCF7 cells were treated with vehicle (DMSO) or 0.4 μM Dox as shown. A Protein was extracted and immunoblotted for E-cadherin, Vimentin, N-cadherin, and Actin. HeLa and MDA-MB-231 cell lysates were used as negative controls for E-cadherin expression and positive controls for Vimentin and N-cadherin expression. B Twist1 expression was analyzed by qRT-PCR using actin as reference gene. HeLa cells were used as a positive control. Data is expressed as mean normalized expression ± SEM (n = 3). C For analysis of the CSC population, cells were stained with FITC-CD24 and PE-CD44 antibodies and analyzed by flow cytometry. MDA-MB-231 cells were used as positive control. D Quantification of CD44⁺/CD24^low/− population in veh/Dox-treated MCF7 cells with MDA-MB-231 cells as positive control (n = 4)