Fig. 5
The different versions of the MMTV-PyMT breast tumor model have distinct immune transcriptional signatures. Tumors from the autochthonous MMTV-PyMT model were harvested and single-cell suspensions were generated. Cells (1E6, 1E5, or 1E4) were injected into the mammary fat pad of FVB/NJ wild-type mice. When tumors reached 100 mm3, tumors were obtained, and RNA was extracted from tumors to assess transcriptional changes. The NanoString PanCancer Immune Profiling Panel was used to analyze gene expression patterns related to the immune cell compartment of the tumors. a A heatmap of all cell-type scores is shown. b Quantification of cell types scores of immune cells, T cells, and macrophages are shown. c Hierarchically clustered heatmap showing the pathway scores across the four models. Yellow and blue represent upregulated and downregulated scores, respectively. d Signature scores from the following pathways are highlighted: inflammation, adaptive immunity, T cell function, innate immunity, dendritic cell functions, macrophage functions, MHC, and antigen processing. e Normalized log2 counts are shown for the following transcripts: Cd274 (PD-L1), Ctla4, Ccr2, and Ccl2. Graphs show mean ± standard deviation with 4–5 mice per group. Each data point represents an individual mouse. Unpaired two-tailed t test. *P<0.05, **P<0.01, ***P<0.001, and ****P<0.001