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Fig. 3 | Breast Cancer Research

Fig. 3

From: Comparing syngeneic and autochthonous models of breast cancer to identify tumor immune components that correlate with response to immunotherapy in breast cancer

Fig. 3

T cell immune subsets differ significantly between the different versions of the MMTV-PyMT breast tumor model. Tumors from the autochthonous MMTV-PyMT model were harvested and single-cell suspensions were generated. Cells (1E6, 1E5, or 1E4) were injected into the mammary fat pad of FVB/NJ wild-type mice. When the tumors reached 100 mm3, tumors were obtained and processed into a single-cell suspension for immunophenotyping by flow cytometry. ad Analysis of T cell subsets as a percent of CD3+ cells and include: a T-regulatory cells (FoxP3+CD4+), b FoxP3-CD4+, c CD8+, and d other CD3+ cells. e Analysis of lymphocyte cell subsets as a percent of viable cells represented as pie charts. f The ratio of CD4+ to CD8+ out of total lymphocytes. g Analysis of T regulatory cells represented by FoxP3+ of CD4+ cells. h The ratio of CTLs represented by GZB+ of CD8+. i The ratio of CTLs to T-regulatory cells. Graphs show mean ± SEM from at least two independent experiments with at least three mice per group. Each data point represents an individual mouse. Unpaired two-tailed t test. *P<0.05, **P<0.01, ***P<0.001, and ****P<0.001

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