Fig. 3

T cell immune subsets differ significantly between the different versions of the MMTV-PyMT breast tumor model. Tumors from the autochthonous MMTV-PyMT model were harvested and single-cell suspensions were generated. Cells (1E6, 1E5, or 1E4) were injected into the mammary fat pad of FVB/NJ wild-type mice. When the tumors reached 100 mm³, tumors were obtained and processed into a single-cell suspension for immunophenotyping by flow cytometry. a–d Analysis of T cell subsets as a percent of CD3⁺ cells and include: a T-regulatory cells (FoxP3⁺CD4⁺), b FoxP3^-CD4⁺, c CD8⁺, and d other CD3⁺ cells. e Analysis of lymphocyte cell subsets as a percent of viable cells represented as pie charts. f The ratio of CD4⁺ to CD8⁺ out of total lymphocytes. g Analysis of T regulatory cells represented by FoxP3⁺ of CD4⁺ cells. h The ratio of CTLs represented by GZB⁺ of CD8⁺. i The ratio of CTLs to T-regulatory cells. Graphs show mean ± SEM from at least two independent experiments with at least three mice per group. Each data point represents an individual mouse. Unpaired two-tailed t test. *P<0.05, **P<0.01, ***P<0.001, and ****P<0.001