Fig. 2
Tumor-infiltrating leukocyte populations differ significantly in the different versions of the MMTV-PyMT breast tumor model. Tumors from the autochthonous MMTV-PyMT model were harvested and single-cell suspensions were generated. Cells (1E6, 1E5, or 1E4) were injected into the mammary fat pad of FVB/NJ wild-type mice. When the tumors reached 100 mm3, tumors were obtained and processed into a single-cell suspension for immunophenotyping by flow cytometry. a Analysis comparing the absolute number of lymphocytes (CD3+) and lymphocyte subsets (CD8+ and CD4+) per gram of tissue. b Analysis comparing the frequency of lymphocytes (CD3+) and lymphocyte subsets (CD4+ and CD8+) out of total CD45+ cells. c Analysis comparing the absolute number of myeloid cells (CD11b+) and myeloid cell subsets (F4/80+ and Gr1+CD11b+) per gram of tissue. d Analysis of myeloid cell subsets as a percent of total CD45+ cells. Graphs show mean ±SEM from at least two independent experiments with at least three mice per group. Each data point represents an individual mouse. Unpaired two-tailed t test. *P<0.05, **P<0.01, *** P<0.001, and **** P<0.001