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Fig. 1 | Breast Cancer Research

Fig. 1

From: Comparing syngeneic and autochthonous models of breast cancer to identify tumor immune components that correlate with response to immunotherapy in breast cancer

Fig. 1

The number of cancer cells inoculated in preclinical models influences tumor growth kinetics as well as the tumor microenvironment. a Tumors from the autochthonous MMTV-PyMT model were harvested and single-cell suspensions were generated. Cells (1E6, 1E5, or 1E4) were injected into the mammary fat pad of FVB/NJ wild-type mice. When the tumors reached 100 mm3, mice were randomized into an experimental group. b Tumor inoculum was generated on three separate days, and flow cytometry was performed to identify the cell proportions. c Tumor volumes were measured every 3–4 days until individual tumors reached 100 mm3; tumor volumes are plotted as average tumor burden ± SEM. dj Tumors were obtained when they reached 100 mm3, and flow cytometry was performed. d Proportion of major cell types are shown. e Percent of CD45+ cells out of total viable cells. fh Proportion of CD3+, CD11b+, and other CD45+ cells of total viable cells. i Ratio of CD3+ to CD11b+ out of viable cells. j Regression analysis of the proportion of CD3+ out of viable cells, to time for tumor to reach 100 mm3. Graphs show mean ±SEM from at least two independent experiments with at least three mice per group. Each data point represents an individual mouse. *P<0.05, **P<0.01, and ***P<0.001

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