Fig. 4

Tumor removal reduces the frequency of circulating CD117⁺ granulocytic cells. A Comparative visualization of the expression of surface markers in monocytes at different time-points of treatment by tSNE. B Heat map of the FlowSOM clustering of breast cancer patients at indicated time-points. Frequency of C monocytes and D granulocytes populations in patients at the indicated time-points during treatment. Frequency of E CD163⁺ and F CD117⁺ granulocyte population at indicated time-points relative to frequency at 0_PreOp time-point. Frequency of the G combined 16 + 18 + 21 + 12 + 15 + 26 and H 1 + 6 + 7 atypical cell populations during treatment. WBC, white blood cells. Cell analysis and quantification was performed by flow cytometry with FlowJo software and results are represented as mean values +/− SD