Fig. 2

Altered frequency of circulating lymphocyte populations in cancer patients. A Heat map of the FlowSOM clustering between breast cancer patients (BC) and healthy donors (HD). tSNE visualization of B the lymphocyte expression profile and C the differentially expressed clusters in the blood breast cancer patients at the time of the first diagnosis of healthy donors. Frequency of the atypical populations D 29 + 24, E 3 + 7, and F 20 at the same timing. WBC, white blood cells. Cell analysis and quantification were performed by flow cytometry with FlowJo software and results are represented as mean values +/− SD