Fig. 4

In vivo drug response to BYL719 or fulvestrant alone and combined in the HBCx-22 TamR and BC1111 PDX models. a Effect of the different treatments on HBCx-22 TamR tumor growth. Each treatment included 10 mice; the y-axis indicates the mean of RTV + SD. b Effect of the different treatments on HBCx953 tumor growth. c, d PLA was performed and analyzed as in Fig. 2. e, f RT-QPCR was performed from RNA extracted from frozen tumor samples using specific primers for ERG. g, h IHC staining was performed on formalin-fixed paraffin-embedded PDX tumors using anti-ERα, anti-P-AKT (S473), and anti-P-S6 riboprotein (S235/6) antibodies. Quantification of highly, medium, and negative cells was performed as described in the “Materials and methods” section. Significance (P value) between treatments and controls were performed using the t test. ns: not significant