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Fig. 1 | Breast Cancer Research

Fig. 1

From: Novel mechanism for OSM-promoted extracellular matrix remodeling in breast cancer: LOXL2 upregulation and subsequent ECM alignment

Fig. 1

Co-expression of OSM and LOXL2 leads to drastically decreased metastasis-free survival. a Distant metastasis-free survival (DMFS) plotted from de Vijver et al. [58] invasive ductal carcinoma patient microarray database comparing low OSM/ low LOXL2 to low OSM/high LOXL2, high OSM/low LOXL2, and high OSM/high LOXL2 mRNA expression (n = 295). We observed a stronger negative impact on DMFS with high OSM and LOXL2 co-expression compared to high expression of OSM or LOXL2 separately (log rank test). b LOXL2 mRNA expression Z-score is positively correlated as measured by Pearson correlation coefficient to the beta subunit expression of OSM receptor (OSMR) mRNA expression Z-score in cancer patients analyzed from The Cancer Genome Atlas (TCGA) RNA-Seq database, specifically: breast cancer (BRCA), glioblastoma (GBM), prostate cancer (PRAD), and ovarian cancer (OV). Scatter plot consists of Z-score mRNA expression and line of best fit as determined by linear regression; a summary of the data is found in the accompanying table. c The mRNA Z-score of several LOXL2 family members exhibit positive Pearson correlation to OSMR mRNA Z-score in the breast invasive carcinoma dataset from TCGA. d qRT-PCR analysis of MCF7 luminal A invasive ductal carcinoma cells treated with OSM shows LOXL2 mRNA induction starting at 12 h and peaking at 24 h; there is no induction with IL-6. e qRT-PCR analysis of MDA-MB-468 basal A invasive ductal carcinoma cells treated with OSM also shows an increase in LOXL2 mRNA expression starting at 4 h. f qRT-PCR analysis of MDA-MB-231 basal B breast cancer cells, that constitutively express high levels of LOXL2, show no significant induction of LOXL2 mRNA expression by either OSM or IL-6 signaling. (All qRT-PCR experiments n = 3+; n.s. p > 0.05, **p < 0.01, ***p < 0.001; two-way ANOVA)

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