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Fig. 3 | Breast Cancer Research

Fig. 3

From: Functional consequences of a rare missense BARD1 c.403G>A germline mutation identified in a triple-negative breast cancer patient

Fig. 3

BARD1 p.Asp135Asn mutation impaired the DNA damage-induced association with RAD51 in vitro. a Western blot (WB) verification of the effect of BARD1MT (p.Asp135Asn) on BARD1-BRCA1 binding before and after irradiation (2 Gy). FLAG-BARD1-WT/FLAG-BARD1-MT and SFB-BRCA1 were co-transfected into T47D and HEK293T cells and applied to immunoprecipitation followed by Western blot. Whole-cell lysates were also blotted and shown as input. b Western blot verification of the effect of BARD1-MT on BARD1-RAD51 binding in T47D and HEK293T cells. Cells expressing BARD1-WTres and BARD1-MTres were treated with irradiation (2 Gy) and then subjected to immunoprecipitation and Western blot

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Breast Cancer Research

ISSN: 1465-542X

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