Fig. 2

BARD1 p.Asp135Asn mutation increased cell sensitivity to DNA damage in vitro. a Western blot testing of the Flag-tagged BARD1 expression in BARD1 knockout (KO), wild-type BARD1^WT rescue (WTres), and BARD1¹³⁵ rescue (D135Nres) breast cancer cell lines. b Clonogenic survival of T47D cells (left) and MDA-MB-468 (right) cells expressing BARD1^WTres or BARD1¹³⁵res after γ-irradiation (IR, up), and cell survival (CCK8 assay) of T47D cells (left) and MDA-MB-468 cells (right) expressing BARD1^WTres or BARD1¹³⁵res after treatment with mitomycin C (MMC, middle) and PARP inhibitor (HS-10160, down). Data are means ± s.d, n=3. P values were calculated using a two-tailed Student’s t-test. *P < 0.05, **P < 0.01