Fig. 7

The effect of attenuated TGFB signalling in macrophages on mammary gland cancer susceptibility in mice. Mammary tumour latency was monitored following DMBA administration in doxycycline-treated Cfms-rtTA, TetO-TbrII and Cfms-TbrII mice (n = 19–20/gp). Mice were palpated weekly, commencing 1 week after the final DMBA dose, with the zero time point representing mice 12 weeks of age. Tumour latency was determined as the time to the first appearance of palpable mammary tumour. The censored mice (mice were killed because of other tumours or sickness) were represented by a vertical line on the plot. Data are presented as Kaplan-Meier tumour-free survival plot (a). Significant difference observed by log rank analysis (Cfms-rtTA vs. Cfms-TbrII; p < 0.05 and TetO-TbrII vs. Cfms-TbrII; p < 0.05). Examples of F4/80 (b–e) and CCR7 (f, g) staining in tumours classified as glandular adenocarcinoma (b, c), carcinosarcoma (d, e) and solid adenocarcinoma (f, g), in tumour samples from Cfms-rtTA (b, d, f), TetO-TbrII (g) and Cfms-TbrII (c, e) mice