Fig. 1

Attenuation of TGFB signalling in macrophages from Cfms-TbrII transgenic mice. Representative immunofluorescent staining of the mammary glands with F4/80 (red channel; a, d, g) and pSMAD2 (green channel; b, e, h) antibodies, and merged images showing co-localisation and DAPI (blue channel) nuclear stain (c, f, i) from single transgenic control Cfms-rtTA (a–c) mice, TetO-TbrII (d–f) mice and double transgenic Cfms-TbrII (g–i) mice. Co-localisation was quantified and expressed as a percent of F4/80-positive macrophages that are pSMAD2 positive (n = 4/gp; j). The spleens collected from doxycycline-treated single transgenic control Cfms-rtTA, TetO-TbrII and double transgenic Cfms-TbrII mice, were analysed for ΔTgfbrII mRNA by RT-PCR and normalised to Actb expression (n = 12–13/gp; k). The result is given in arbitrary units where the average of the single transgenic control Cfms-rtTA is 1. Data are presented as mean + SEM with statistical analysis using a Kruskal-Wallis test, *p < 0.05, ***p < 0.005