Fig. 5
Immunohistochemistry of MCF10ADCIS lesions. Serial histological sections (a) of one representative MCF10ADCIS lesion resected 8 weeks post-intraductal cell implantation. H&E, Ki-67, CC3, TUNEL, and C2Am/iC2Am ratiometric fluorescence signals are shown. Chart (a), correlation of the C2Am/iC2Am ratiometric signal with the percentage of cell death, determined by TUNEL assay, for regions of interest (ROI) of the lesion shown in a. L, lesion; fp, fat pad; sk, skin. Correlation of C2Am/iC2Am ratiometric signal (b) from multiple lesions, fat pad, and skins from several mice with the corresponding levels of TUNEL staining. C2Am/iC2Am ratiometric signal (c) in multiple fat pad, skin, and lesions. L1: < 10% TUNEL positivity, L2: 10–25%, L3: 25–50%, L4: > 50%. b, c, n = 10 mice, 89 lesions, 22 fat pad, 17 skin samples. c Two-tailed, t test, unequal variance. **P < 0.005, ***P < 0.001, ****P < 0.0001. Scale bars (a) = 2 mm