Fig. 2
Detection of MCF10ADCIS cell death in vitro using C2Am. Cells were treated with chemotherapeutic drugs (doxorubicin or etoposide) and incubated with either an equimolar mixture of C2Am|iC2Am or with a fluorescent inhibitor of effector caspases (FLICA) as a reference gold standard. Plates (a) were scanned at different excitation/emission wavelengths: iC2Am (650/680 nm), C2Am (780/800 nm), and FLICA (450/480 nm). Correlations of iC2Am staining (b), C2Am staining (c), and the ratio C2Am/iC2Am (d) with FLICA staining. UT-untreated (open circles), DOX-doxorubicin (inverted triangles), and ETP-etoposide-treated (filled circles) cells. C2Am and iC2Am were labeled with DyLigh-750 and AlexaFluor-650, respectively (see Supplementary Figure S1 and Methods). FLICA-fluorescent inhibitor of effector caspases. n = 4 technical replicates per experimental condition, 2 independent experiments