Fig. 5

Identification of miR-3613-3p target genes and related signaling pathway. a Venn diagram of predicted target genes of miR-3613-3p from Targetscan, miRWalk and miRTarBase online websites. b Schematic diagram of 3′-UTR dual-luciferase reporter system. c Predicted 12 target genes of miR-3613-3p were verified by 3′-UTR dual-luciferase reporter assays in HEK293T or MCF-7 cells (*P < 0.05). d The expression of SMS, PAFAH1B2, or SNHG16 was shown in two separate group samples (MIR3613 deletion group or MIR3613 non-deletion group) of breast cancer tissues from TCGA database. e Transcripts of differentially expressed genes were analyzed in two groups of breast cancer patients divided according to MIR3613 CNVs from TCGA dataset (group of MIR3613 CNV ≥ 0, n = 586; group of MIR3613 CNV < 0, n = 492), and enriched top five signaling pathways (blue) were shown. Transcripts of differentially expressed genes were analyzed in two groups of breast cancer patients divided according to SMS, PAFAH1B2, and PDK3 expression from TCGA dataset (group of 3 target genes high expression, n = 166; group of 3 target genes low expression, n = 197), and enriched top five signaling pathways (orange) were shown. f Transcripts of differentially expressed genes were analyzed in two groups of breast cancer patients divided according to SMS, PAFAH1B2, and PDK3 expression from TCGA dataset (group of 3 target genes high expression, n = 166; group of 3 target genes low expression, n = 197). The expression of CDC6, CDC25A, CDK1, ATM, E2F1, and MKI67 in above two groups were shown according to the transcriptome sequencing data