Fig. 3

miR-3613-3p suppressed malignant phenotype of human breast cancer cells. a Proliferation of MCF7 cells transfected by miR-3613-3p mimic or control mimic was analyzed by CCK-8 assay (***, P < 0.001). b Apoptosis of MDA-MB-231 cells transfected by miR-3613-3p mimic or control mimic was analyzed by flow cytometry. The representative apoptosis images were visualized on the left and the proportion of apoptosis cells were statistically analyzed on the right (**P < 0.05). c The expression of miR-3613-3p in non-spheres or spheres of MDA-MB-231 cells was calculated by RT-PCR (**P < 0.05). d Tumor spheres of MDA-MB-231 cells transfected by miR-3613-3p mimic or control mimic was cultured with serum-free medium for 7 days. The representative tumor sphere images were visualized by microscope on the left (scale bar, 200 mm) and the sphere number per field were statistically analyzed on the right (**P < 0.05). e Transcripts of differentially expressed genes were analyzed in two groups of breast cancer patients divided according to MIR3613 CNVs from TCGA dataset (group of MIR3613 CNV ≥ 0, MIR3613 non-deletion, n = 586; group of MIR3613 CNV< 0, MIR3613 deletion, n = 492). The expression of SOX2, NANOG, and LIN28B in above two groups were shown according to the transcriptome sequencing data